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Where is Helene ? (French accent waring + weird voice) - Duration: 0:12.
For more infomation >> Where is Helene ? (French accent waring + weird voice) - Duration: 0:12. -------------------------------------------
Ben is Under Uma's Spell - Part 30 - Descendants in Avalor Disney - Duration: 6:08.
Disney Doll Story
Elena.
I am the new queen of Avalor.
Do as I say.
I will rule Avalor with absolute power.
I will go and plunder everything in Avalor.
No.
You can't do this.
Of course I can.
Half of plunder is mine.
Evil spirit.
Change him right now.
Uma.
No.
Why isn't this working?
Ben.
What's wrong with you?
Don't hurt him.
You got what you wanted.
No, move over.
Ben.
What's this?
What are these stones?
Mateo is already giving out the stones to everyone in Avalor.
You can't have Avalor.
What?
Ben.
Evil spirit, get him.
No.
No.
Maleficent, you haven't done anything weird to Audrey, did you?
Well.
oh, a few harmless harmless curses.
More like gifts.
Oh, Maleficent.
What curses did you put on Audrey?
You don't have to know.
Audrey, wake up please.
Audrey, are you alright?
Evie?
Evie.
Oh, I was afraid I will never wake up.
Audrey, you are going to help me get the sceptor.
Wait.
I changed.
Audrey, please.
Let's go.
Yes.
Ben.
I will do everything Uma says.
Say it.
No.
Spirits.
Get him.
He still hasn't changed.
Get them out of my head.
Uma, stop this.
I'm not afraid to use my power.
Let's do this.
My sceptor versus your magic.
No.
Please.
No.
If both of you fight, there wouldn't be any Avalor left.
You give up Ben first.
Ben?
Ben.
Get up.
Mal.
Don't do this.
Ben.
Are you alright?
Mal.
Give up.
There is no winning this.
Tell her how great I am.
Mal, you should accept Uma as your new queen.
Ben.
No.
Uma, what should I do with Mal?
Ben, please tell me.
You are just pretending.
Ben, make her listen to me or I will have to get rid of her.
Audrey, you are going to help me get Uma's sceptor.
How?
This is poison.
You want me to poison Uma?
Well, she's already poisoned.
Then why do you need to poison her again?
I need to cure Uma, but there is no cure for the other poison.
I don't understand.
No time to explain.
I need you to go and make her drink this.
But why me?
Because she wouldn't drink it if I give it to her.
Ben.
I finally saw the truth.
Ben.
What truth?
Uma is the future.
Mal, you are too weak.
Ben.
Mal.
Uma made me see it.
You can't even remember me.
Ben.
I will remember.
I will remember
Mal.
I have to help Uma.
I hope you accept Uma as your new queen.
Ben.
No.
No.
We have to get in.
Nobody out here can open up the barrier.
Is that Mal?
Mal.
What happened?
Lonnie.
Did I really love Ben?
I can't remember anything.
Of course you did.
Uma was right.
Maybe I pretended to love him.
No, it was a real love.
I don't think I could've loved him.
Oh, Mal.
You have to trust me.
You love Ben a lot.
And so does he.
Mal.
Lonnie.
Gabe.
Audrey?
You are alright.
I was worried Maleficent might have done something to you.
No.
Mal.
What happened?
Ben and Mal fought.
As in they broke up.
Oh, Mal.
You and I share something in common now.
He dumped me too.
I don't want to talk about it.
No, Mal.
We can't let him get away this time.
Open up this barrier for me
Audrey?
What are you planning to do?
I come bearing a gift.
Poison from Evie.
Let's go Mal.
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The Alphabet Is So Much Fun! | Kids Songs | Super Simple Songs - Duration: 2:05.
A...B...C...D...
E...F...G...
H...I...J...K...
L M N O P
Q...R...S...
T U V
W...X...Y...and Z
A...B...C...D...
E...F...G...
H...I...J...K...
L M N O P
Q...R...S...
T U V
W...X...Y...and Z
The alphabet is so much fun
I love to sing with everyone
Now I know my ABCs
Next time won't you sing with me
A...B...C...D...
E...F...G...
H...I...J...K...
L M N O P
Q...R...S...
T U V
W...X...Y...and Z
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ONLY 1 CUP IS ENOUGH FOR LOSING STOMACH FAT! - Duration: 1:19.
ONLY 1 CUP IS ENOUGH FOR LOSING STOMACH FAT!
THE NECESSARY INGREDIENTS ARE:
4 lemons
3 honey tablespoons
2cm fresh ginger
125g horseradish
2 cinnamon tablespoons
THE PROCESS OF PREPARATION IS THE FOLLOWING:
� With an electric mixing machine, mix the ginger and horseradish.
� The juice of the squeezed lemons put it in the mixture of the ginger and horseradish.
� Continue mixing for additional 3 minutes.
� Put the honey and cinnamon and continue blending until you see that the syrup has
been made.
� Put the syrup in a glass jar.
HOW TO USE THE SYRUP:
� Before you eat your meals or before you go exercise you will consume 1 teaspoon two
times in the day.
� The right consummation of this syrup is three weeks
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Celebrity Doppelgangers : Who is Who? #Stolk - Duration: 4:23.
For more infomation >> Celebrity Doppelgangers : Who is Who? #Stolk - Duration: 4:23. -------------------------------------------
New Results - Grown up sales techniques - Milk is for closers - Duration: 0:35.
For more infomation >> New Results - Grown up sales techniques - Milk is for closers - Duration: 0:35. -------------------------------------------
Videoflash n°18 - What is co-production? - Duration: 1:54.
Hi.
I hope that your summer break was as fascinating and relaxing as mine.
In Brussels and Strasbourg, the work of European institutions started again.
Many discussions are planned with regard to the funding of Social Services
and how to fund innovation in our field. We keep an eye on these debates.
And also, the very important debate with regard to the Accessibility Act, that is planned in the
European Parliament, it is a very important one. We keep you posted!
Take now a quick look at a message from our policy team. Bye!
Hello. Support Services face many challenges nowadays.
It's important to keep a vision of how to adapt and how to evolve
to face future challenges.
One of the models is co-production.
According to co-production, individuals, like person with disabilities,
have to be meaningfully engaged throughout the process
and they have to participate in the design of services,
implementation, financing and every part of it.
In that way, the service
is more adaptable to the challenges that will face in the future.
EASPD, together
with EDF, ENIL, Inclusion Europe, Mental Health Europe and COFACE Families Europe
is involved in a taskforce that tries to come together and find a
common understanding of challenges and how we can work together to bridge those issues.
It's not always easy work, but we think
it's vitally important to bring all stakeholders together at the table to find
sustainable solutions.
We think it also could be a very good solution for your services.
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Jesu Wakanaka (Jesus is good) - True Worshippers - Duration: 1:47.
Somebody make a joyful noise unto the Lord!
Jesu wakanaka, hiya (Jesus is good, yeah )
wakanaka, hiya ( is good, yeah )
wakanaka, hiya, hiya, hiya, hiya, hiya, hiya ( is good, yeah, yeah, yeah, yeah, yeah, yeah ) ehhh!
Jesu wakanaka, hiya (Jesus is good, yeah )
wakanaka, hiya ( is good, yeah )
wakanaka, hiya, hiya, hiya, hiya, hiya, hiya ( is good, yeah, yeah, yeah, yeah, yeah, yeah )
hiya, hiya, hiya, hiya, hiya, hiya ( yeah, yeah, yeah, yeah, yeah, yeah )
Ehhh! Jesu anerudo, hiya (Jesus has got love, yeah)
Anerudo, hiya (has got love, yeah)
Anerudo, hiya, hiya, hiya, hiya, hiya, hiya (has got love, yeah, yeah, yeah, yeah, yeah, yeah)
Ohhh! Jesu anerudo, hiya (Jesus has got love, yeah)
Anerudo, hiya (has got love, yeah)
Anerudo, hiya, hiya, hiya, hiya, hiya, hiya (has got love, yeah, yeah, yeah, yeah, yeah, yeah)
hiya, hiya, hiya, hiya, hiya, hiya ( yeah, yeah, yeah, yeah, yeah, yeah )
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Destiny 2 gameplay #1 Is he a gundam or bane ? which one ! - Duration: 52:41.
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What is the PAM? - A CRISPR Whiteboard Lesson - Duration: 5:04.
What is the PAM?
A CRISPR White Board Lesson presented by the IGI.
Editing genomes with CRISPR proteins involves something that often confuses people
a little sequence called the PAM.
What is a PAM?
Why does it exist?
And why does it matter?
We're going to help you understand.
It all starts with what CRISPR systems originally evolved to do: defend bacteria against viruses.
Bacteria face the constant threat of infection and destruction by a special type of virus
- the bacteriophage.
If bacteriophages are able to inject their DNA genomes into the bacterial cell, replicate
inside, and burst out, the cell is toast!
In response, bacteria have evolved a protective immune system called CRISPR.
The CRISPR array is a short stretch of DNA in bacteria composed of alternating repeated
sequences and target-specific spacers.
These spacers contain the DNA of invading viruses collected from past infections.
When a virus infects a bacterium, a new spacer is added in to the growing CRISPR array.
This process begins when a protein complex, known as Cas1 and Cas2, identifies the invading
viral DNA, and cuts out a segment of a specific length.
This segment of DNA is known as the protospacer.
The protospacer is inserted into the front of the CRISPR array.
Now the bacteria has an embedded memory of this phage infection.
If the phage returns, the bacteria is armed to defend itself.
This defense starts with transcribing a long CRISPR RNA (crRNA) from the repeats and spacers
of the CRISPR array.
Another RNA called the trans-acting or tracrRNA comes along and links up with the crRNA through base pairing
A protein, known as Cas9, grabs onto the dual RNAs, and they're trimmed to a more manageable
size to form a complete search complex.
If the sequence of the crRNA matches the sequence of the invading virus, Cas9 cuts the viral
DNA and destroys the phage, allowing the bacterial cell to survive.
*Snip! *Snip!
But wait… the viral DNA that is targeted by the search complex is the exact same sequence
as the DNA in the CRISPR array, so how exactly is Cas9 able to distinguish between itself
and the enemy?
This is where the PAM comes in.
The PAM, which stands for the protospacer adjacent motif, is a specific sequence of
nucleotides, around 2–6 base pairs, that follows the protospacer sequence in a viral genome
In Streptococcus pyogenes, Cas9 recognizes the PAM sequence "GG," with an additional
nucleotide between it and the protospacer.
This PAM sequence must be present for the Cas9 protein to know that it's okay to latch
onto and cut this region of DNA.
How does this keep the bacterium from hurting itself?
The key is that the spacer sequences within the CRISPR array are NOT followed by a "GG."
The sequence of the repeat is always the same -"GTT."
This means that the Cas9 is unable to bind to the CRISPR array and thereby avoids cutting
the bacterium's own genome.
But how is it that there's always a PAM sequence at a true Cas9 target?
In the DNA acquisition step we covered previously, we mentioned that the Cas1–Cas2 protein
complex is in charge of capturing new spacers from incoming viral DNA.
Cas9 works with Cas1 and Cas2 to find a PAM sequence and remove the protospacer next to it.
Only picking targets with PAMs guarantees that when the same virus infects again and
Cas9 is armed with a matching crRNA guide, nothing will stop it from destroying the enemy DNA.
The PAM sequence also serves an additional role.
Searching through all the DNA inside a bacterial cell can take a very long time, but the PAM
sequence accelerates the search process.
Instead of trying to unwind every bit of DNA to check for a match, Cas9 bounces around
the cell, searching for a tiny PAM sequence.
If it finds one, only then does it check to see if the crRNA matches.
So how is the PAM involved in Cas9 genome editing?
If scientists want to use Cas9 to cut human DNA or the DNA of any other organism, they
first look for a PAM sequence within the target genome and then design an RNA to match the
sequence next to it.
But what if there is no "GG" next to what scientists want to cut?
Fortunately, "GG" isn't the only PAM in town.
Scientists can edit genes with Cas9s from different organisms and even different CRISPR proteins
These different proteins have all evolved to recognize distinct PAMs.
Scientists have even engineered the S. pyogenes Cas9 to recognize other PAMs.
The future of genomics is in our hands, so make sure not to forget the PAM!
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Arie Luyendyk Jr. Is The New Bachelor! - Duration: 4:23.
Arie Luyendyk Jr, may not be a name that fans of The Bachelor will recognize immediately, but its one theyve heard before. The 35-year-old racecar driver appeared on the eighth season of The Bachelorette in 2012.
After Emily Maynard chose Jef Holm in the series finale, Arie lobbied to be the next Bachelor, but producers repeatedly chose someone else for the gig.
Now, after five years of waiting, Arie is finally getting his chance to be the man handing out the roses on the long-running ABC reality series. The surprising decision was revealed today on Good Morning America.
I just kind of went in with an open mind, open heart, Luyendyk told Michael Strahan in a live interview. It just kind of hit me right now that I'm here.
Luyendyk said the whole process happened so rapidly that he hasnt even had a chance to speak with his family about his decision. It's been such a quick turn of events and my family doesn't know, said Luyendyk.
So now they know. So they know along with you guys.. Luyendyks efforts to woo Maynard earned him the nickname the Kissing Bandit during his previous stint with the franchise.
Asked if he would be engaging in more lip-locking on the upcoming season of The Bachelor, Arie said fans will just have to wait and see. Obviously there's got to be that chemistry there, he said.
Emily and I had it previously and I'm hoping to find that type of love, for sure.
While the show doesnt have the greatest track record in terms of creating lasting relationships, Luyendyk says hes 100 percent sincere in his search for Ms. Right.
I'll be 36 in a few weeks so for me it's time, he said of finding love.
All my friends around me, you know, have kids and have grown up, so for me, I feel like I'm the last one of my friends to really take that step.
Though Luyendyk was a fan favorite on The Bachelorette, fans were somewhat surprised that he was chosen by producers for a second shot at love.
Insiders say that as recently as last week, producers were in full-blown panic mode, as they struggled to find a suitable Bachelor who was up for the job.
The past few months saw candidates dropping like flies, as Dean Unglert revealed he was not ready for a relationship, thus taking him our of the running. Shortly thereafter, Peter Kraus bowed out, leaving showrunners with few options.
When it was revealed that Eric Bigger wouldnt be the Bachelor either, fans began to express shock and confusion on social media.
So Arie may not be producers first choice for the job, but were guessing the Kissing Bandit will prove up to the task.
Watch The Bachelor online at TV Fanatic to get caught up in time for whats sure to be an amusing season.
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