Thứ Hai, 30 tháng 1, 2017

Waching daily Jan 31 2017

Hey, atleast it's not a bottle-flipping theory...

For more infomation >> Totally Legit Game Theory: Sans is Steven? - Duration: 0:19.

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Hamed Abdel Samad There is no peace in Islam and Islam's main goal is to dominate the whole world - Duration: 2:09.

Concerning the infidels ( in Quran 9:5)

whom even you ( Muslims) have a covenant of peace with them

a truce

there is no peace anymore !

and this shows that there is no peace in the generally understood meaning of the word in Islam

or a complete peace

the peace in the islamic point of view is that any country should be subjugated under the rule of Islam

and ruling should be exclusively under the control of Muslims

and that's called '' the house of salaam'' ( the house of Islam )

there is another house called '' the house of war '' which is any place in the world that's Non-Muslim

so , in other words, this is an agenda of islamic conquests

that's what the house of war means

For more infomation >> Hamed Abdel Samad There is no peace in Islam and Islam's main goal is to dominate the whole world - Duration: 2:09.

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"The Wizard is back" | Terraria Montage | W/ Tiger9712 | - Duration: 2:44.

I'm just holding my binding around

hoping already a lot holy crap well

there's a lot that's a lot and a lot of

good cup of tea ok i was carrying too

much do you think I up and it's we're

just don't happen to know where

hello everybody today we're playing

Terraria tiger and this is like my

second time playing not really but I

still a minus and I don't know what I'm

doing

you want what you want

ok so hope you guys enjoy if you like

this thing to do as a serious except i'm

a lazy person so yeah and i hope you

guys enjoy subscribe and comment down

below what you want to see next

hope you guys enjoy it and you guys all

next time ok goodbye

For more infomation >> "The Wizard is back" | Terraria Montage | W/ Tiger9712 | - Duration: 2:44.

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LET THE BIBLE SPEAK - The Unpardonable Sin - Duration: 28:15.

- What is the unpardonable sin?

Find out next on Let the Bible Speak.

♪ Music ♪

Announcer: From the churches of Christ

Let the Bible Speak

with Evangelist Kevin Presley.

I'm so glad you've joined me today

for a study of the Word of God.

One of the most common questions that preachers are asked,

I suppose, is the question

what is the sin that God will not forgive?

You know, the Bible speaks about that.

And can men commit that sin today,

or, more personally, have I committed the unpardonable sin?

Most students of the Bible surely have wondered

about the words of our Lord recorded

in Matthew Chapter 12, beginning in verse 31.

There, Jesus said, "Wherefore I say unto you,

"all manner of sin and blasphemy

"shall be forgiven unto men,

"but the blasphemy against the Holy Ghost

"shall not be forgiven unto men.

"And whosoever speaketh a word against the Son of man,

"it shall be forgiven him,

"but whosoever speaketh against the Holy Ghost,

"it shall not be forgiven him, neither in this world,

"neither in the world to come."

Serious words indeed,

and whatever this seemingly mysterious sin

against the Holy Spirit is,

we dare not be found guilty of it.

In our study today,

we'll look at this weighty passage of Scripture

and learn about the unpardonable sin.

- If you've ever visited an Assembly of the Church of Christ

you've seen that it's different.

No rock bands, no choirs and praise teams,

no theatrical productions.

That's because we believe worship is simple

but profound, and is according to what's revealed

in God's Word.

When you visit with the Church of Christ,

you'll find that everybody simply sings

the praise of the Lord together, congregationally.

We meet around the Lord's table every Sunday

to remember the body and blood of the Lord

in His new covenant.

We pray together, and none of that pop psychology.

But sound teaching from the Word of God.

Oh and one more thing.

We won't ask for your money.

Members provide for the needs of the local church

through a weekly collection.

So forget all the hype.

Come see the difference,

and be our honored guest today.

Any sin is serious in the sight of God.

By definition, sin is the transgression

or breaking of God's law,

according to 1 John 3 in verse 4.

And all sin is devastating to our relationship to God

who is holy and just.

The prophet Ezekiel said in Ezekiel 18 in verse 20,

"The soul that sinneth, it shall die."

And Paul declared that, "The wages of sin is death,"

Romans 6 in verse 23.

But the Bible also tells us that God is rich in mercy

and eager to forgive our sins when we approach Him

through Christ in obedient faith,

and any sin that a man can repent of can be forgiven,

and any person who can believe and obey

the conditions of the gospel can be saved.

Jesus made that clear in Matthew 11 in verse 28.

He said, "Come unto Me all ye that labor

"and are heavy laden and I will give you rest."

When the Lord commissioned His apostles

to preach the gospel in Mark 16, beginning in verse 15,

He said, "Go into all the world,

"and preach the gospel to every,

"every creature.

"He that believeth and is baptized shall be saved."

And just before the pen of Revelation

was laid down forevermore,

as if to leave us with one last assurance

of God's grace and limitless mercy,

John wrote in Revelation 22 in verse 17,

"The Spirit and the bride say, 'Come.'

"And let him that heareth say, 'Come.'

"And let him that is athirst come.

"And whosoever will,

"let him take the water of life freely."

Those who are Christians are told in 1 John 1 in verse 9,

"If we confess our sins,

"He is faithful and just to forgive us our sins

and to cleanse us from all,"

A-L-L, "all unrighteousness."

But yet we have the text of our study today

in Matthew chapter 12.

And there, the Scripture seems to strike a different tone

when Jesus said that all manner of sin would be forgiven,

even one who speaks against God's own Son, Jesus Christ.

But one sin, He says,

is beyond the reach of God's forgiveness,

and that is the blasphemy of the Holy Spirit.

Jesus said that one who blasphemes or speaks against,

is what the word blaspheme means,

one who speaks against the Spirit of God

would not be forgiven then

nor in the world that was to come.

Well, those words ring with cold

and hopeless finality, don't they?

No forgiveness ever.

Once you've committed that sin,

according to Jesus, it's over.

We also read in Hebrews chapter 6

that there are those who,

having tasted of the Good Word of God

and the powers of the world to come and then fall away,

that it is impossible to renew them under a penance,

"seeing they crucify to themselves the Son of God afresh,

"and put him to an open shame,"

Hebrews chapter 6 and verse 6.

Well, these passages seem to suggest

that some sins are of such a nature

that they are beyond the reach of God's mercy.

For some reason and in some respect,

those sins are considered, well, unforgivable.

So which is it?

Can one be cleansed of all unrighteousness, as John says?

Or are there sins that are well beyond the pale

and cannot be forgiven?

Well, Paul told Timothy that,

"All Scripture is given by inspiration of God,"

2 Timothy 3 in verse 16,

and God cannot lie, and so therefore,

Scripture does not contradict Scripture.

And the true interpretation of each passage

will harmonize with the others, or else,

one or more of those interpretations is false.

So, what is Jesus saying when He speaks of the sin

that God will not forgive?

Can I commit it today?

Is it possible that you or I are guilty of it?

Well, first, let's consider the usual explanations

given to this passage in Matthew chapter 12.

Some, first of all, think that the sin consists

of attributing Christ's power

to perform miracles to Beelzebub.

Now, it is true, if you look here

at the surrounding context,

that what the Pharisees witnessed

were the miracles of Jesus,

and when they saw His miracles,

they couldn't deny that they had seen a miracle.

They would have been foolish to deny

that a miracle had taken place.

You see, miracles then were real miracles.

They weren't what men today claim are miracles.

There's no disputing that a miracle took place

when one did take place.

And so even the Pharisees couldn't deny

that Jesus had manifested miraculous power.

The problem is it put them on the horns of a dilemma.

They either had to acknowledge

that Christ's power came from God

and thus, they would have to acknowledge

and confess Him to be who He claimed to be, the Son of God.

Or they had to deny the miracle, and they couldn't do that

because it was obvious a miracle took place,

and they refused to believe that Jesus was the Son of God,

so what they did was they said

that His power came from Beelzebub.

It came from an evil source.

Well, that certainly was a sin against the Holy Spirit,

and it certainly involved in what Jesus

was referring to here on this occasion.

But I think we'll see today

that this sin against the Holy Spirit

really has a much wider application.

And then some believe that this sin

could only be committed while Jesus

was on earth performing miracles.

And since the miraculous age has passed,

well, it therefore has no relevance to modern people.

Since the Holy Spirit is no longer empowering men

to perform miracles, then it would be impossible

to sin against Him, as Jesus speaks of.

Well, that can't be true because neither God nor Christ

are empowering men to perform miracles today,

and so that reasoning would lead us to conclude

that one could not sin against Christ or God today either,

so that can't be correct.

And then some would warn us that refusing to accept

the modern and speculative theories that people advance

about the Holy Spirit and His supposed manner

of working in the world today,

well, that might just be the sin that Jesus was speaking of.

But that's not the case either because, listen,

for one thing, rejecting the false doctrines

of men about the Holy Spirit,

that's not speaking against the Holy Spirit.

It's defending the truth of the Holy Spirit.

It's not speaking against the Spirit Himself.

It's speaking against the false doctrines

that these men come up with and spread

about the Holy Spirit that the Bible does not teach.

And then there are those who are under the impression

that the sin against the Holy Spirit

is decreed unpardonable because of the special nature

of the Holy Spirit and His work.

In other words, Christ was really exalting

the third person of the God head

as being, in effect, so holy and special

that it is beyond the pale and unforgivable

to speak a word against Him.

But that has nothing to do with it per se.

Yes, the Holy Spirit is divine,

He is the third person of the God head,

He is certainly holy and to be reverenced,

but no more than Christ or God the Father.

In fact, Jesus said in John 16 in verse 14

that the Spirit's purpose was to bring glory to Jesus,

not the other way around.

You know, listening to some preachers today,

you would almost think that Jesus was sent

to be the forerunner of the Holy Spirit,

but that's not the way it was.

The Holy Spirit came after Jesus

in order to point back to Jesus

and to shed light upon Him

and to lift Him up and glorify Him

by the revelation of the gospel plan

of salvation in Christ.

And then some have concluded that the unpardonable sin

might be the act of suicide, since one who commits it

has no opportunity to repent and seek forgiveness.

But as terrible and heartbreaking of a circumstance

for all involved as suicide may be,

it's not a sin against the Holy Spirit

in the context that Jesus is speaking of here.

Nowhere is such idea or such an action

under consideration in this particular passage.

And finally, some believe that the sin

against the Holy Spirit is the so-called unforgivable sin

that John speaks of over in 1 John 5 and verse 16.

Now that passage reads that,

"If any man see his brother sin a sin

"which is not unto death,

"he shall ask, and he shall give him life for them

"that sin not unto death.

"There is a sin unto death.

"I do not say that he shall pray for it."

Well, now let's think about that for a moment.

John says, now follow it closely, John says

there is sin that is not unto death.

Now that doesn't mean that any sin is not serious

or he is not saying that there are sins

that are not worthy of death.

That's not what he is saying.

He is saying that there is not a sin

that brings irrevocable judgment

for which there is no repentance and no forgiveness.

He says there is sin that is not unto death.

And when a brother commits such a sin,

he says we may pray to God on his behalf,

and the Lord will hear and answer.

But on the other hand,

he says there is a sin that is unto death.

That is, this is a sin of finality.

And he says when a brother is guilty of it,

it does no good to pray for him

because God will not forgive it.

Well, that sounds rather frightening.

What is this sin unto death that John is warning of?

Well, the answer is actually back in chapter 1 of 1 John.

Look again in 1 John 1 beginning in verse 7.

He says, "But if we walk in the light,

"as he is in the light, we have fellowship

"one with another, and the blood of Jesus Christ His Son

"cleanseth us from all,"

A-L-L, "all sin."

And if we say that we have no sin,

we deceive ourselves, and the truth is not in us.

Look at it now.

If we confess our sins, He is faithful and just

to forgive us our sins and to cleanse us

from all unrighteousness.

Now, consider what he says.

This passage here says that the Lord will forgive

all sins a brother confesses.

Well, if he says all sins can be forgiven,

then there is no exception to that.

The Lord will forgive all sins a brother confesses.

But in 1 John 5 in verse 16, he there says

that there is a sin the Lord won't forgive.

Now if the Lord will forgive all sins

that a brother confesses, but on the other hand,

there is a sin the Lord won't forgive,

what we conclude from that is

the sin that the Lord will not forgive is the sin

that a brother refuses to confess or acknowledge.

That is, if a man will not acknowledge his sin

before God and thus repent of it and turn away from it,

it does no good to pray to God for His forgiveness.

He has to repent of his sin.

He has to acknowledge that to God,

humble himself before God and turn from his sin,

or else, there is no forgiveness for it.

Not only that, the sin under consideration

in Matthew 12 and 31 was a sin committed

by an unbeliever who rejected the deity of Jesus.

However, the sin of 1 John 5 and verse 16

is a sin that is committed by a brother in the Lord

who has, at one time, accepted the truth about Jesus.

So they're not talking about the same thing.

The sin that John is contemplating is simply a sin,

is simply a sin that a Christian refuses to acknowledge

and therefore turn from, and it does no good

to pray for such and want forgiveness.

And consequently, that passage refutes

the Calvinistic doctrine of "Once saved, always saved,"

as John is writing a letter to saints or Christians,

and he warns them about a brother

committing a sin unto death.

Well, how can a man be saved if he's guilty of a sin

that God will not forgive, you see.

But back to Matthew 12 now.

Since we've seen what Jesus is not talking about,

let's see what He is talking about.

Let's read the passage again.

He says, "Wherefore I say unto you,

"all manner of sin and blasphemy

"shall be forgiven unto men

"but the blasphemy against the Holy Ghost

"shall not be forgiven unto men.

"And whosoever speaketh a word against the Son of man,"

that is, against Jesus,

"it shall be forgiven him

"but whosoever speaketh against the Holy Ghost,

"it shall not be forgiven him,

"neither in this world,

"neither in the world to come."

Now notice carefully,

a person may sin against God and be forgiven.

He may sin against Christ and be forgiven.

But He says if he sins against the Holy Spirit,

he cannot be forgiven.

And then Jesus adds, "Neither in this world

"nor in the world to come."

Now, the translation "world" there

is perhaps a little misleading to some.

He's not saying in this life nor in the life beyond.

That can't be the case because the fact is

no sin will be forgiven in the life that is to come.

Now is the time of salvation,

the Bible is abundantly clear about that.

There will be no forgiveness extended

after the probation period

of this earthly life is over.

Right now, God's mercy is being shown to mankind

and offering him the gospel plan of salvation.

You see, the judgment bar is not going to be

where God's mercy is shown.

God's mercy is shown now,

and you and I best avail ourselves of it

while we yet have the breath of life

and the power to choose it.

God's mercy is shown now, and if you refuse it,

then only judgment awaits in the life to come.

So what does He mean when He says

"in the world now or in the world to come?"

Well, actually, the word translated "world" in this passage

is often used in the Bible for age or dispensation.

So then what Jesus is saying is

that those who committed this sin against the Holy Spirit

could not be forgiven in the age in which Jesus spoke this,

which was consequently the Mosaic age

living yet under the law,

and neither, He says,

will it be forgiven in the age or dispensation to come,

which is the present age or the Christian dispensation,

when the gospel, here's the key, when the gospel

would be fully preached and offered to all mankind.

So, what is the sin?

Why is it so serious to sin against the Holy Spirit?

Well, Guy N. Woods used to illustrate it this way,

and I think it's an excellent and fitting explanation

and a very logical explanation of the words of Jesus.

He said imagine a man is in a boat floating down the river.

And below him are some dangerous rapids

over which, if he passes, he goes to his death.

And let's say a little way down the river,

a man stands on the bank and shouts a warning to him.

The man in the boat ignores it and floats on.

A little way down, a second man shouts a second warning

and offers to pull the man out of the water.

But let's say he refuses that man too and he floats on.

And just before he reaches the brink,

a third man shouts a final, emphatic, plain warning to him,

makes a last offer to save the man,

but that too is refused.

And so the man is swept over the rushing falls to his doom.

Now let's think about that.

He might have accepted the first man's offer

and have been saved.

Perhaps he waits and accepts the second man's offer.

He could still be saved.

In fact, he could have even rejected

the first and second men's offers

and still accepted offer number three

and still have been saved.

But when he passed up the third offer,

he passed his last chance of salvation.

Well, you know, that's what many have done.

You know, when man sinned in the Garden of Eden,

God's justice would have allowed

the annihilation of the human race.

But mercy stepped in.

Thank God, mercy stepped in and stayed the judgment of God.

And for at least 4,000 years,

through the tearful pleadings of the Old Testament prophets,

God sought to turn wayward men from his sinful course

but with little success.

In fact, the prophet Isaiah represents himself

as "standing with outstretched arms

"before a disobedient and gainsaying people,"

Paul records in Romans 10 and 21.

So through the prophets, God extended the first offer,

which was largely refused.

And then in the fullness of time,

He sent His Son to the lost world.

That was offer number two,

and that too was rejected,

since John 1:11 reminds us that, "He came unto His own

"and even His own received Him not."

Not only did the masses reject Him as the Son of God

but crucified him.

But still, amazingly still,

the mercy of God had not run out.

And just 50 days after the Resurrection on Pentecost,

offer number three was extended

to them and to you and to me.

It was extended through the work

and the influence of the Holy Spirit

that was poured out upon the apostles.

He worked through the apostles

and there revealing the gospel to the world.

But my friend, listen carefully.

That's it.

That's offer number three.

That was the last.

There will never be another plan offered to the world.

The gospel which the Holy Spirit brought to earth

through the apostles and confirmed in them

by the miracles they performed

is the final offer of mercy to mankind.

And if you reject the gospel plan of salvation,

doom awaits, no if, and, or but about it.

Paul declared in 2 Corinthians chapter 1:7-9,

"To you who are troubled rest with us,

"when the Lord Jesus shall be revealed from heaven

"with His mighty angels, in flaming fire

"taking vengeance on them that know not God,

"and that obey not the gospel of our Lord Jesus Christ

"who shall be punished with everlasting destruction

"from the presence of the Lord,

"and from the glory of His power."

Remember the Hebrew writer warned those Christians

who would leave Christ

and return to the sacrifices of the Jewish temple

in Hebrews 10:27-29, saying,

"But a certain fearful looking for

"of judgment and fiery indignation,

"which shall devour the adversaries.

"He that despised Moses' law died without mercy

"under two or three witnesses.

"Of how much sorer punishment, suppose ye,

"shall he be thought worthy,

"who hath trodden under foot the Son of God,

"and hath counted the blood of the covenant,

"wherewith he was sanctified, an unholy thing,

"and hath done despite unto the Spirit of grace?"

Now don't think that this is a sin that's committed

in one single act of disbelief.

It's a gradual sin,

begins when one grieves the Spirit

like a disobedient child grieves a parent.

It leads to one resisting the teaching of the Spirit,

as the Jews did that resisted the prophets.

And finally, quenching the Spirit, that is,

destroying the influence of the Spirit in your life

by totally and finally repudiating

and shutting the door of your heart to the gospel.

And in so doing, the sinner becomes numb

to the appeal of the gospel,

and he becomes so hard of heart

that the gospel cannot reach him.

You see, the unforgivable sin is not a sin

that God cannot forgive

as much as it is a sin that man cannot repent of

because he destroyed his heart through his rejection,

and he passed up the last and final and proven appeal

of salvation in Jesus Christ revealed with the Spirit,

and friend, that's very serious.

And if you have not obeyed the gospel,

I want you to open the door of your heart to Christ today

by obeying Him in baptism,

and I hope you'll do it today.

- The Psalmist said, "Through Thy precepts,

"I get understanding."

The Bible is the revelation of God to man

and you simply can't live for God until you know something

about the Word of God

and you may say, "Well, I want to read

"and study the Bible, but I don't know where to begin.

"I feel overwhelmed" or "I don't understand the Bible."

I want to offer you a wonderful way

to get acquainted with the scriptures.

You'll learn about some of the most basic

and foundational teachings of God's Word

and you'll get a better handle on how to read

and approach and study the Bible as a whole.

Won't you get in touch with us today

and ask to be enrolled in the Bible Correspondence Course.

It won't cost you a penny and we'll mail the lessons

to your home and you take your time to read and study

through the lessons,

I think you'll be surprised how much you'll learn.

- [Voiceover] Want to see today's study again?

Watch Let the Bible Speak any time.

Even on the go.

On your computer, tablet, or smartphone.

Go to letthebiblespeak.tv

and also connect with us on Facebook, Twitter, and YouTube.

If you'd like to go back and study

about the unpardonable sin,

perhaps you'd like a transcript

of the lesson we presented today,

we'll be happy to send you one free of charge.

Get in touch with us with the contact information

we'll provide you in just a moment,

and we'll be glad to mail you a copy.

You can also look up the information on our website,

letthebiblespeak.tv.

You'll find our email address there,

and you'll also find past broadcasts

of Let the Bible Speak.

We're on social media.

Be sure to like us on Facebook, follow us on Twitter.

We're also on Instagram and Snapchat.

And now, you can download the free podcast

of Let the Bible Speak each week.

Just go to iTunes and look for

Let the Bible Speak and subscribe.

We sure hope you'll join us back here next time

for another study of the Word of God,

and we'll look forward

to meeting you here then, if the Lord wills.

Until then, have a great week,

and may the Lord bless you.

For more infomation >> LET THE BIBLE SPEAK - The Unpardonable Sin - Duration: 28:15.

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MSU Denver New Student Orientation is now hiring for 2017 - Duration: 4:15.

New Student Orientation is hiring for 2017 and..

We want you to join the Orientation team

I just got word the road runners celebrities are on their way

come on, lets see

And it looks like they've finally arrived, the road runner celebrities

Now that we've seen the road runner celebrities walk the red carpet,

Lets turn to Denny for answers to the questions you've been dying to hear

Well I didn't realize there was a huge body of students

that actually knew me but then it hit me when

one of my theatre friends told me he had heard this

awesome and funny story about me from

one of his friends that transferred into here

Working with new student orientation has changed my life

in such a positive way

I honestly never saw myself being in the place I am today

confidence has always been an issue for me

I've always been too scared to speak up

and being a leader never passed into my head until I

Started working with New Student Orientation

Working with new student orientation has developed my leadership skills and

made it so I'm more adapt to learning from my peers and supervisors

and learning which types of leadership styles work with different types of people

I'm continuously growing in not letting fear get in the way

of tasks that need to be completed

I always feel like I have a group of people that support me no matter what the situation is

I really like the experience that the job provides and the

many different skills you learn that you can apply elsewhere

for example I'm studying to become a highschool teacher and

and having groups of students has really influenced me and how I want to interact with my future students

I love getting to help students, I like seeing them succeed and

I love seeing them get excited for coming to MSU Denver

Glamorous

Adventurous

Diversity

Support

Family

Energetic, WHOOOO

Join the N-S-O family and unlock YOUR leadership skills

For more infomation >> MSU Denver New Student Orientation is now hiring for 2017 - Duration: 4:15.

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Recalibration & DNA Upgrade In Preparation Of The New Moon - Duration: 5:20.

Recalibration & DNA Upgrade In Preparation Of The New Moon.

by Tiffany Stiles,

Dear Beautiful Souls!

Today appetites will be null as the body detoxes and continues to clear.

All goes offline and back online as this recalibration occurs.

The body physically will be achy today as further clearing occurs through the heart,

throat and sacral chakras.

The palpitations and flutters in these chakras makes you aware when expansion/clearing is

occurring.

As one fully begins to integrate with the higher vibrational energies of gamma, photon,

star particle and crystalline, the body requires rest, plenty of water and self care.

Every particle, atom and cell rejuvenates with these creating transcendence of lower

vibrational energy to higher within.

As your body continues to upgrade in frequency with these downloads of energy, the higher

frequencies enact a firing off of the DNA within upgrading your DNA�s spliced strands

recalibrating and recoding.

This fires off signals in the brain connecting new pathways that weren�t enabled before.

As the gamma, photon, star particle surges through the body it affects the nervous system

as everything recalibrates and comes back online.

As this occurs, one is also upgraded with new Metaphysical abilities and capabilities

as further mass in the brain connects to the upgraded DNA/RNA.

When this occurs you have high vibrational frequency surging through your body, this

affects electronics you work with or are in contact with.

You will notice these will also come online and go off line too.

This includes your cell, laptop, tablet, TV, radio, vehicle, lights, etc�

You will have the frequencies coming in through the ears with ringing, high pitched vibration

and humming.

This is how new information is downloaded and given.

Quiet the mind to hear your messages from your Guides.

The lungs are clearing attached to the heart chakra.

You may have had extra mucous expel through the night.

Headaches also may be experienced throughout the day as all adjusts within.

You may notice extra water retention too indicating a flush of toxins needing released.

Do this through extra water to release through the kidneys and bladder attached to the sacral

chakra.

The 3rd Eye (pineal) over produces melatonin and fluctuates serotonin to force the rest

the body needs, this also causes moods to fluctuate.

DMT also peaks with visions through rest/REM or meditation.

Journal these.

The Crown becomes tingly, itchy, and tight while it opens to receive Divine Energy through

and in.

Here you connect with your Angels, Guides, Archangels/God Source/ Ascended Masters/ Goddesses.

The root chakra with kundalini becomes active and rises.

Your solar plexus activates your free will.

When clearing occurs here is causes gastrointestinal issues and stomach upset.

This also activates the GPS of the soul, your inner guidance/intuition.

All chakras are firing up through these upgrades and this clearing, releasing, activating,

recalibrating is what enacts the DNA upgrades/crystalline light body.

You Become A Conduit

You can feel this energy surging through your hands and feet, with your pulse throbbing

at your palms and fingertips.

This is your body acting as a conduit for this energy to flow through to heal others,

anchor into Gaia, and send out into the collective consciousness to stabilize, calm, transmute,

transcend.

Through this pure state of unconditional love, compassion and empathy connected to All That

Is, you are an alchemist.

You have the capability to tap into the Divine Energy flow and manifest whatever you wish.

Keep your thoughts positive.

Today, as the body goes offline to recalibrate and adjust your energy will be low.

Take today to rest as the next major shift comes in on the 26-28th with New Moon in Aquarius.

The Twin Flame/Soul Mate Portal is open now and further gamma, photon and star particle

will come in on these days too to activate/release more.

See That Energy Report By Clicking Here!

Will update as we continue to move through this

next powerful shift.

For more infomation >> Recalibration & DNA Upgrade In Preparation Of The New Moon - Duration: 5:20.

-------------------------------------------

North Korea more likely to test mid-range Musudan missile, rather than ICBM: Seoul military official - Duration: 0:54.

North Korea is more likely to test fire a Musudan intermediate-range ballistic missile

before it tests a long-range one.

That's according to a South Korean military official, who said North Korea is more likely

to focus on increasing the reliability of the Musudan... as an intercontinental ballistic

missile launch would certainly end in failure.

Last year the North test-fired eight Musudan and only one was deemed a success.

North Korean leader Kim Jong-un said in his New Year's address that his country is almost

ready to test-fire an ICBM, but Seoul interpreted it as a move to attract the attention of the

Trump administration.

But the military official warned that an ICBM launch is not entirely off the table, because

ultimately a launch depends on the whim of the North Korean leader.

For more infomation >> North Korea more likely to test mid-range Musudan missile, rather than ICBM: Seoul military official - Duration: 0:54.

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What is Globalization? - Duration: 8:26.

What is globalization?

Our textbook answers the question by saying that globalization

is a convergence of five things. I'm not going to distract you with wobbly or spinning

graphics after that. I just wanted to wake you up. Globalization is the convergence of 5 things. First it's a convergence of economies.

Back in the middle of the 20th century we can describe national capitalisms. So there were

capitalist countries but they were fairly autonomous from each other

in the sense that they were regulated by their governments, they have different laws that protect a different industries. They were semi-

independent from each other. Yes there was international trade between them but

still it was a world in which each capitalist country was its own system.

Now more and more we are in an age when there is simply one worldwide capitalist free market.

People talk about

the global free market in which laissez faire capitalism occurs. Laissez faire simply means to leave something alone.

Don't interrupt it. Don't put in regulations or rules or customs that interfere with the pure

functioning of supply and demand economics, which is pure capitalism.

So the world is becoming more and more one big open free market stage for capitalism.

And within the world economy we have the appearance of global financial institutions.

These are things like the World Bank. The world has a bank. Countries go to the bank and borrow

money from it. And they have to pay the money back with interest.

The World Bank lends money, often vast sums of money to countries

in an attempt to help them develop. Most of the loans are specifically designed to help developing countries

improve their infrastructure somehow so they can become more competitive in this world free

market system. So the World Bank actively promotes free market capitalism

and lends money to countries trying to get them

to succeed in that free market.

Another global financial institution here is the International Monetary Fund which is very similar to the World Bank. It also loans money.

However, when the IMF loans money it attaches strings to the loan.

So the IMF says all right, I'll lend you money, but if you take the loan you have to change your policies in order to make your country

more free market-oriented. What that usually means is the country is obliged to cut social spending

so they can't use money on education and health care and things like that as much because they

need that money to repay the loan with the interest.

They also have to get rid of tariffs and other taxes that interfere with laissez faire capitalism.

And other things that we'll be talking about in this class.

The World Trade Organization is another global financial institution. Most countries in the world have signed the World Trade Organization

treaty - they're members -- and the WTO regulates the free trade that's going on in this world free market.

Transnational corporations like McDonald's are are global financial institutions. These are companies that are so global

that they more or less don't have a home country at all. They simply float around the world. They have branches, shops, restaurants whenever

whatever, all around the world. So they really have a home country. They just float around the earth.

Uh.

And there's a shoe, which is kind of a symbol of the global free market. This is the Air Max Penny which is the favorite example

from your textbook. To make an Air Max Penny 120 different workers have to touch it.

It has in its ingredients that make it come from all around the world. The cotton may come from Brazil;

The leather er the rubber might come from Peru; the metal grommets if there are any might come from South America;

and on and on. So it's taking materials from around the world, passing through many many workers hands, ultimately coming together in

this one shoe which is then sold in the global free market through Amazon.com or what have you.

Globalization is also a convergence of ecosystems. As I just said, I am now going to repeat myelf. Here we can even see,

It's like mining, you know maybe the grommets are coming from here; rubber is being tapped from this tree.

Uh, here is more mining going on somewhere and here is cotton being harvested all to produce this one item which then is sold

worldwide. So the production of products draws together materials taken from ecosystems

around the world. And they converge in the form of these products

and sold on the world market.

Another thing that's converging is information. There are almost no parts of the world anymore that are shrouded in mystery and darkness and

the unknown because there's somebody there with a cell phone and they're uplinking information to the Internet.

So we can now see information from everywhere around the world. Of course not all

parts of the world are equally Internetted and can do this.

But there's somebody even in a country like North Korea which is

highly censored and regulated area nobody can get in and out freely. But there is still somebody smuggling in a cell phone.

And we can see what's going on. That's why some people think

there will be the ultimate good effect of the convergence of information will be some kind of

universal standard for human rights. Because we all see what was going on around the world.

There's no part of the world where customs that are so out of the ordinary and different

can take place where we don't know about. So hopefully the fact that we all know what's going on will kind of lead us toward formulating a

universal standard of human rights. Maybe that's optimistic but it's a hope.

People are converging.

When we meet in class have a look around the room. The Cal State Northridge student body are made up of people have come here

from around the world. Many of them or their families just within the last generation or two.

So Los Angeles is a great example of a place - a world city -

that draws people from all around the world. So globalization involves the convergence of people in specific spots like world cities.

And language convergence. This is the last one. So increasingly, even though people may have their own first language they are

increasingly required to speak a second language that that is the universal second

language. The language of business that people speak around the world.

Let me let you read my funny cartoon here.

So this guy on the left probably speaks Japanese as a native, the guy on the right might speak Swahili, but the language they have in

common is English. And yes, English is the common international language.

A long time ago Germany was sort of playing that role and seemed like German might become the the world's second language.

And that's why the term English now is lingua franca - Frankish tongue. English has replaced

Germany as the international business language.

And

that's it about globalization for now. Thank you.

For more infomation >> What is Globalization? - Duration: 8:26.

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Video: Tom Messner is looking at a cold night. 1.30.17 - Duration: 4:04.

TURNER,

REPUBLICAN.

REPUBLICAN. >> NOW, FIRST ALERT WEATHER WITH

>> NOW, FIRST ALERT WEATHER WITH TOM MESSNER.

TOM MESSNER. TOM: YOU PROBABLY NOTICED IT IS

TOM: YOU PROBABLY NOTICED IT IS COLDER TODAY THAN THE WEEKEND.

COLDER TODAY THAN THE WEEKEND. IT WAS NICE TO SEE THE SUN.

IT WAS NICE TO SEE THE SUN. THIS AREA OF HIGH PRESSURE WILL

THIS AREA OF HIGH PRESSURE WILL HANG AROUND TOMORROW.

HANG AROUND TOMORROW. THE CLOUDS WILL THICKEN.

THE CLOUDS WILL THICKEN. WE WILL HAVE SNOW ON THE WAY.

WE WILL HAVE SNOW ON THE WAY. MAYBE AN INCH ON WEDNESDAY.

MAYBE AN INCH ON WEDNESDAY. THE SYSTEM IS TO THE WEST.

THE SYSTEM IS TO THE WEST. THE INCH IS IN THE CHAMPLAIN

THE INCH IS IN THE CHAMPLAIN VALLEY.

VALLEY. IT WILL TAKE MORE OF THIS TO GET

IT WILL TAKE MORE OF THIS TO GET HERE.

HERE. THAT MEANS WE HAVE SUNSHINE IN

THAT MEANS WE HAVE SUNSHINE IN THE FIRST PART OF THE DAY.

THE FIRST PART OF THE DAY. MAYBE LONGER THAN THAT.

MAYBE LONGER THAN THAT. THEN IT WILL CHANGE IN THE

THEN IT WILL CHANGE IN THE AFTERNOON.

AFTERNOON. ANOTHER SHOT OF WHAT WE ARE

ANOTHER SHOT OF WHAT WE ARE DEALING WITH.

DEALING WITH. WE DO NOT HAVE PROBLEMS LOCALLY.

WE DO NOT HAVE PROBLEMS LOCALLY. WE HAVE SOME CLOUDS.

WE HAVE SOME CLOUDS. THE ODD SNOWFLAKE IS A

THE ODD SNOWFLAKE IS A POSSIBILITY.

POSSIBILITY. THIS PART OF THE CLIPPER SYSTEM

THIS PART OF THE CLIPPER SYSTEM IS MOVING OUR WAY.

IS MOVING OUR WAY. IT WILL NOT LAST.

IT WILL NOT LAST. IT WILL DROP A LITTLE BIT OF

IT WILL DROP A LITTLE BIT OF SNOW.

SNOW. NOT A TON OF MOISTURE.

NOT A TON OF MOISTURE. IT WILL BE DIFFERENT TOMORROW.

IT WILL BE DIFFERENT TOMORROW. CURRENT TEMPERATURES ARE 18 IN

CURRENT TEMPERATURES ARE 18 IN BURLINGTON, 20 IN PLATTSBURGH,

BURLINGTON, 20 IN PLATTSBURGH, IT IS COLDER AND SINGLE DIGITS

IT IS COLDER AND SINGLE DIGITS IN THE LAKES.

IN THE LAKES. TO THE SOUTH, WE HAVE COLDER AIR

TO THE SOUTH, WE HAVE COLDER AIR NOT QUITE YET.

NOT QUITE YET. LEBANON IS 28.

LEBANON IS 28. 27 IN SPRINGFIELD.

27 IN SPRINGFIELD. RUTLAND IS AT 25.

RUTLAND IS AT 25. HEADING TO TOMORROW AND THE END

HEADING TO TOMORROW AND THE END OF THE WORK WEEK, WE WILL BE AT

OF THE WORK WEEK, WE WILL BE AT 23 TOMORROW, THE AVERAGE HIGH IS

23 TOMORROW, THE AVERAGE HIGH IS 27 AND 28.

27 AND 28. WE WILL BE DEAD ON, ON

WE WILL BE DEAD ON, ON WEDNESDAY, AND WE DIP DOWN.

WEDNESDAY, AND WE DIP DOWN. WE WILL SEE SUNSHINE ON SOME OF

WE WILL SEE SUNSHINE ON SOME OF THE DAYS.

THE DAYS. TOMORROW AT 8:00 IN THE MORNING,

TOMORROW AT 8:00 IN THE MORNING, IT WILL BE SUNNY.

IT WILL BE SUNNY. IN THE CHAMPLAIN VALLEY, SUNNY

IN THE CHAMPLAIN VALLEY, SUNNY AND 18.

AND 18. IN THE 20'S IN THE AFTERNOON.

IN THE 20'S IN THE AFTERNOON. THE SYSTEM TO THE WEST IS

THE SYSTEM TO THE WEST IS AFFECTING US IN THE AFTERNOON

AFFECTING US IN THE AFTERNOON COMPARED TO EARLIER.

COMPARED TO EARLIER. 7:30 TONIGHT, A FEW CLOUDS.

7:30 TONIGHT, A FEW CLOUDS. IF YOU HAVE A BREEZE, IT WILL

IF YOU HAVE A BREEZE, IT WILL NOT LAST.

NOT LAST. THE WIND WILL PULL BACK.

THE WIND WILL PULL BACK. IT WILL BE QUIET TOMORROW.

IT WILL BE QUIET TOMORROW. IT WILL BE COLD.

IT WILL BE COLD. WE HAVE SUNSHINE.

WE HAVE SUNSHINE. WE WILL GET YOU IN THE

WE WILL GET YOU IN THE AFTERNOON.

AFTERNOON. WE WILL FIND MORE CLOUDS IN THE

WE WILL FIND MORE CLOUDS IN THE AFTERNOON AND MAYBE SNOW SHOWERS

AFTERNOON AND MAYBE SNOW SHOWERS TO THE SOUTH.

TO THE SOUTH. PARTICULARLY IN THE MOUNTAINS.

PARTICULARLY IN THE MOUNTAINS. AND CLOUDY LATER IN THE DAY IN

AND CLOUDY LATER IN THE DAY IN THE CHAMPLAIN VALLEY.

THE CHAMPLAIN VALLEY. WE WILL FIND LIGHT SNOW AND SNOW

WE WILL FIND LIGHT SNOW AND SNOW SHOWERS OVERNIGHT IF NOW BEFORE

SHOWERS OVERNIGHT IF NOW BEFORE ON WEDNESDAY.

ON WEDNESDAY. WE HAVE ACCUMULATION OF AN INCH

WE HAVE ACCUMULATION OF AN INCH OR SO IN THE CHAMPLAIN VALLEY

OR SO IN THE CHAMPLAIN VALLEY AND MORE IN THE MOUNTAINS.

AND MORE IN THE MOUNTAINS. WEDNESDAY NIGHT, THOUGH, IT WILL

WEDNESDAY NIGHT, THOUGH, IT WILL BE PULLING OUT.

BE PULLING OUT. RIGHT NEW, THURSDAY, GROUNDHOG

RIGHT NEW, THURSDAY, GROUNDHOG TAG -- DAY IS GOOD.

TAG -- DAY IS GOOD. TONIGHT, CLOUDY AND LIGHT WINS.

TONIGHT, CLOUDY AND LIGHT WINS. TEMPERATURES ARE FIVE IN

TEMPERATURES ARE FIVE IN PLATTSBURGH, AND SEVEN IN

PLATTSBURGH, AND SEVEN IN BURLINGTON, AND SARANAC LATE AT

BURLINGTON, AND SARANAC LATE AT TWO BELOW BUT WARMER IN LEBANON

TWO BELOW BUT WARMER IN LEBANON AND SPRINGFIELD AND RUTLAND AT

AND SPRINGFIELD AND RUTLAND AT TEN DEGREES.

TEN DEGREES. TOMORROW WE REBOUND.

TOMORROW WE REBOUND. PARTLY SUNNY.

PARTLY SUNNY. I WOULD CALL IT COOL AT 23,

I WOULD CALL IT COOL AT 23, BURLINGTON AND PLATTSBURGH, 24

BURLINGTON AND PLATTSBURGH, 24 IN SPRINGFIELD.

IN SPRINGFIELD. NEWPORT IS TOPPING OUT TOMORROW

NEWPORT IS TOPPING OUT TOMORROW AT 21.

AT 21. 23 TOMORROW.

23 TOMORROW. MORE CLOUDS AROUND BY THE

MORE CLOUDS AROUND BY THE AFTERNOON.

AFTERNOON. SNOW SHOWERS ACCUMULATING ON

SNOW SHOWERS ACCUMULATING ON WEDNESDAY AT 28.

WEDNESDAY AT 28. GROUNDHOG DAY ON THURSDAY, IT

GROUNDHOG DAY ON THURSDAY, IT WILL BE 23.

WILL BE 23. SUNNY ON FRIDAY, BUT WEEKEND IS

SUNNY ON FRIDAY, BUT WEEKEND IS LOOKING OKAY.

LOOKING OKAY. IT WILL BE CHILLY PARTLY SUNNY

IT WILL BE CHILLY PARTLY SUNNY SATURDAY AND SUNDAY.

SATURDAY AND SUNDAY. THE HIGH ON SATURDAY IS 19.

THE HIGH ON SATURDAY IS 19. 23 ON SUNDAY.

For more infomation >> Video: Tom Messner is looking at a cold night. 1.30.17 - Duration: 4:04.

-------------------------------------------

This is how a TakuWood is Shaped - Duration: 1:28.

With the help of this machine, the TAKU WOOD is shaped into the designed..,

For more infomation >> This is how a TakuWood is Shaped - Duration: 1:28.

-------------------------------------------

2064: Read Only Memories' Starfucker Is Only Rated Teen - Duration: 2:00.

Hello.

This is Jonathan from Censored Gaming.

We have already gone over the different ratings Root Letter has in both North America and

Europe, with how certain curse words made the game rated M, while in Europe, it was

rated with a PEGI 12.

This showed how some countries were lenient with the rating.

This included Australia and Germany, who can be stricter with games like this, not giving

it a rating equivalent of a M rating in North America.

However, a recent post in the Censored Gaming subreddit revealed something stranger with

2064: Read Only Memories.

It only received a T for Teen rating, releasing on PlayStation 4 and Xbox One on January 17th

of this year, but released on Steam on October 6, 2015.

This is despite the fact that there are two instances of strong language being used.

The first is a character's name being Starfucker.

The game also features voice-acting, actually saying the name, so this isn't fully dependent

on just the writing for swearing.

The second instance is when ordering a drink, in which it is called a Fuckin' IDK.

These were the only two examples provided, but seemed extreme enough that it does bring

up why Root Letter was given an M, yet Read Only Memories got away with a T. Even the

developer proudly showed off a screenshot for the game.

It's unknown if this was done through an update.

Considering the original Steam version in 2015, it seems unlikely, especially when the

screenshot posted on Reddit was taken on a PlayStation 4.

Nor does anything on the developer's Twitter, or the Twitter for the game itself, reveal

that this was done in any update.

Uses of the F word are said to give games an instant M rating by ESRB, so it's a possibility

that this is something that the rating system somehow managed to overlook.

Should more information become available, we will keep you updated.

For more infomation >> 2064: Read Only Memories' Starfucker Is Only Rated Teen - Duration: 2:00.

-------------------------------------------

This field is auto filled - Duration: 2:57.

For more infomation >> This field is auto filled - Duration: 2:57.

-------------------------------------------

Parking at city-owned garages is about to be more expensive - Duration: 1:51.

IN CITY GARAGES, THERE'S ABOUT

TO BE A DISTURBANCE IN THE

TIME-PARKING SPACE CONTINUUM.

ADD TO THE EQUATION, A RATE

INCREASE.

WHAT'S YOU'RE REACTION WHEN YOU

HEAR THIS?

WHAT?

--

WHAT?

BRIAN: THAT'S YOUR REACTION.

>> IT REALLY IS.

SAY WHAT?

I'M TELLING YOU I WAS REALLY

SHOCKED WHEN YOU MENTIONED THAT.

BRIAN: BEGINNING IN MARCH, THE

MAJORITY OF CINCINNATI OWNED

PARKING GARAGES WILL INCREASE

THEIR PARKING RATES.

MOST MONTHLY PARKERS CAN EXPECT

TO PAY BETWEEN $10 AND $15 MORE

PER MONTH, AND HOURLY RATES WILL

ALSO SEE AN INCREASE.

SO THE CITY OWNS EIGHT PARKING

GARAGES AND SIX OF THOSE ARE

GOING TO SEE INCREASES.

>> CORRECT.

BRIAN: ROCKY MERZ IS THE

SPOKESMAN FOR THE CITY.

HE SAYS THE PLAN IS TO BRING

CITY RATES CLOSER TO WHAT

PRIVATE LOTS ARE CHARGING.

>> IN JUST ABOUT ALL OF THESE

LOCATIONS, YOU'RE STILL GOING TO

GET A CHEAPER RATE AT THE CITY

GARAGE.

BRIAN: THE MONEY GOES INTO A

DEDICATED FUND THAT IS FOR

MAINTENANCE AND IMPROVEMENTS TO

PARKING.

>> WHAT WE'RE DOING NOW IS

ADJUSTING THOSE UP TO MAKE SURE

THAT WE HAVE THE CORRECT AMOUNT

OF RESOURCES COMING IN TO MEET

THE MARKET DEMAND.

BRIAN: AT THE CITY OWNED

GARFIELD GARAGE, THERE'S MIXED

REACTION.

>> I WORK AT SKYLINE, SO I'M ALL

TIPS.

SO DEPENDS ON THE DAY HOW MUCH I

MAKE, AND SOME DAYS IT'S NOT

GOOD, AND ALL MY PAY GOES TO

PARKING, SO THAT KIND OF SUCKS,

YEAH.

>> MAINTENANCE HAS TO BE DONE.

PEOPLE HAVE TO BE PAID

LIGHTS HAVE TO STAY ON

ELEVATORS NEED MAINTENANCE AND

WORK.

THAT'S REASONABLE.

BRIAN: FOR NOW, PARKERS HAVE A

LITTLE OVER A MONTH TO BRACE FOR

PARKING TO GO UP ANOTHER LEVEL.

THE CITY SAYS THEY'RE OVERDUE

FOR A PARKING HIKE.

THEY HAVEN'T RAISED THE RATE

SINCE 2010.

For more infomation >> Parking at city-owned garages is about to be more expensive - Duration: 1:51.

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Core Group Realty: How we determine a home's fair market value - Duration: 2:39.

(pulsing electronic music)

- Well, hi there, Thom Dallman here with Core Group Realty.

In our line of business we get asked quite often

from our clients how we determine that

a house is actually listed at a fair market value.

We do that in several different ways.

First off, we actually like to pull

the sold comps in the area

and really look closely at the homes in the area

that have sold in the last three to six months,

compare them, we compare, you know, number of rooms,

square footage, garage size,

amenities such as granite counter tops, wood floors,

things like that, so we really get a really good idea,

a comparison on the homes that are in the same area,

the same general vicinity,

that sold in the last three to six months.

To make sure that the pricing is accurate for

fair market values in today's marketplace,

and the reason that we do this is because

when we go to submit an offer on behalf of a client,

we want to make sure they're submitting a fair price,

that they're getting what they feel is a good deal,

but also to make sure that there's no issues

when it comes time for an appraisal.

You know, down the line through the loan process

we're going to have to have an appraiser come out,

the lender will, send in an appraiser to go

make sure that the house is being bought

at fair market value, and so we want to make sure

that we're as close as possible to that mark,

because if an appraiser comes in and says that

the house is not of the value of what the offer is,

we can run into some problems.

There's usually some negotiating that can happen

at that point, either the sellers will drop the price

to where the fair market value is, or

the buyer has to come up with some extra cash

to purchase the house if they really want it, so

there's things that can be worked around

if an appraisal comes in less, but

that's why we ask agents and why it's very important

to have a trusted real estate agent helping you

in that process, really taking a look at those comparables

in the marketplace.

Hope this bit of information has been helpful for you.

Don't forget that if you need any help in

getting your home value or in looking at homes,

we have a couple of convenient links down below,

our home search site where you can get all the MLS listings,

updated every 15 minutes for you, as well as

a home valuation page where you can go

and put in your address and find out

what your home's current value is in today's marketplace,

it gives you a kind of general idea.

Hope you're all having a wonderful day,

and thanks a lot for listening.

(pulsing electronic music)

For more infomation >> Core Group Realty: How we determine a home's fair market value - Duration: 2:39.

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Proper PCR Cleanup before Sanger Sequencing - Seq It Out #12 - Duration: 3:09.

Why is a clean template for your Sanger sequencing reaction important?

And what are the ways to get it without losing too much precious samples?

Let's find out today.

The sequencing workflow usually starts with PCR also known as Polymerase Chain Reaction,

where the target fragment is replicated into thousands of copies under a controlled condition

using primers, DNA polymerases and deoxynucleotides.

Before the PCR product is ready for Sanger sequencing, you must do one more thing, a

PCR clean up step.

Because Sanger sequencing is a highly accurate technique for you to read DNA sequence base

by base, it is very important to clean up your reaction mixtures so that those unincorporated

primers and dNTPs won't interfere with your results.

So how do you perform this PCR clean up step and what are your options?

There are several methods for PCR cleanup such as ethanol precipitation, bead or column

based purification, and Enzymatic approaches.

Ethanol precipitation is most cost effective but is also the most labor intensive.

Bead or column based purification can be an effective method for many applications.

However, this route tends to be a bit pricier compared to other options.

This method of purification also requires transferring samples in and out of the column

which can often result in the loss of some of your precious samples so this may not be

the best method for those with limited starting materials.

An enzymatic cleanup approach has a really simple and straightforward workflow.

In fact, it's only one single pipetting step!

It is also relatively affordable compared to the bead or column based purification methods.

Basically, you add an enzyme mix to your finished PCR reaction and let it sit at a certain temperature.

But to understand this method better, let's take a look at our lab book.

One of the enzymes, exonuclease I(one), digests excess primers.

At the same time the other enzyme in the mix, alkaline phosphatase, dephosphorylates nucleotides

in the reaction.

Dephosphorylation means that the enzyme renders the dNTPs from your reaction useless, so that

they don't disturb the sequencing reaction.

This is important because the sequencing reaction has a very specific ratio for its nucleotides

and you don't want to change that ratio.

Now the enzymes have done their work and you don't need them anymore.

You can simply heat up the reaction mix to 80 degrees and this will deactivate the enzymes.

The result is a very clean amplicon that is now ready to go into your sequencing reaction.

You don't need to perform any other steps.

And you didn't lose any of your template either.

You are done!

But I am sure you'll have more questions on PCR cleanup.

Submit your question at thermofisher.com/ask and subscribe to our channel to see more videos

like this.

But remember, when in doubt, just Seq It Out.

For more infomation >> Proper PCR Cleanup before Sanger Sequencing - Seq It Out #12 - Duration: 3:09.

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'Alien' life could exist high in Earth's atmosphere - Duration: 4:41.

�Alien� life could exist high in Earth�s atmosphere.

by Chuck Bednar.

What if alien organisms are hiding in plain sight, high above the planet�s surface in

our own atmosphere? It�s a question that researchers will attempt to solve later on

this year, and it may have implications on how we search for microbial life on other

planets as well.

According to Seeker, a team of students from the University of Houston plan to launch a

high-altitude experiment as part of a mission to study auroras that is scheduled to lift

off from Alaska in March. Their experiment, which the website said �looks almost like

a small laundry hamper,� will search for microbes in the atmosphere 11-31 miles (18-50

km) from the ground.

Once it reaches its target altitude, the instrument will open up to collect samples, then close

as the balloon-equipped experiment begins to descend. The students believe that this

system will be less likely to become contaminated than more complicated systems, but since it

has not yet been tested, they are not 100% certain exactly how well it will perform in

high altitude conditions. �A lot of times, these microbes when they

go up there, they shut down. They are not replicating and they are not metabolically

active,� Houston student and project team member Jamie Lehnen told Seeker on Friday.

�I�m interested in how their stress response is similar to those [microbes] back on Earth's

surface."

Research could impact search for life on other planets

Roughly two months after the Houston team�s experiment, NASA plans to conduct a study

of their own: the ABoVE (Arctic-Boreal Vulnerability Experiment), which according to Seeker is

set to run in May and June and will involve using a Gulfstream III jet to monitor how

changing climate conditions are affecting plants, animals and the environment as a whole.

David Smith, a microbial researcher at the US space agency, will fly with the ABoVE team

to study the microorganisms that travel in a massive spring airstream that move along

the Pacific Ocean. As he told the website, he and his colleagues hope to find out what

organisms are being carried across the ocean along with the dust and aerosol particles

moved by that airstream.

The research project �will allow us an opportunity to test the atmospheric bridge hypothesis,

which simply speaking, is continents sneezing on each other,� Smith explained, telling

Seeker that he and his fellow researchers plan to use an instrument called a cascade

sampler to collect samples from high altitudes. The cascade sampler allows air to pass through

increasingly finer impact plates designed to trap out dust and small particles, which

can then be studied later.

Smith said that his team hopes to find microorganisms �persisting� and surviving, as it is rather

unlikely that they are growing or dividing due to the extremely cold and arid conditions

at such a high altitude. Scientists have never previously measured how long microbial lifeforms

are able to remain alive in the stratosphere, he added, calling that �work that still

needs to be done.�

Should they actually discover microbial life lingering, or even growing and dividing, at

such high altitudes, it could have an impact on the search for life on other planets as

well, including Venus. Earth and Venus similar for a period of three billion years, noted

NASA astrobiologist Dr. Lynn Rothschild, a member of Smith�s team. Experts have discovered

an unusual entity in the atmosphere of Venus that blocks ultraviolet light, and living

organisms have not been ruled out as a possibility.

For more infomation >> 'Alien' life could exist high in Earth's atmosphere - Duration: 4:41.

-------------------------------------------

Eat Your Celery and Get Many Great Benefits: Celery Does 3 Things Really Well! Watch to Learn - Duration: 2:41.

Celery health benefits and what your body will do with it, hi my name is David Claussen with quick nutrition for busy people

and I did some research online to find out some benefits and one from MNT that's huge is that celery will help lower your blood pressure

so your heart and cardiovascular health is going to be happier right away with the celery in the body according to Dr. Axe

a big one is that it lowers inflammation and inflammation is often a contributing cause of

diseases like cancer heart disease arthritis and more celery is gonna help you with

many types of those pains from arthritis

inflammation can give you joint pain as gaut kidney and liver infections

and other skin disorders

and when you chew on a celery stock it's also the helpful

with your liver

and your liver is one of your largest organs and it is important for many metabolic functions because it converts the nutrients in our diets

to substances that your body can use so you really want a healthy liver and celery is one of the seven most alkaline foods

and alkaline helps level that ph or the acid

in your body because you want that chemical balance in your body

so your body can function at a high rate so make sure you eat as much celery as possible for your body

and if you're looking for the best foods in the world I've compiled a free grocery list in a video so that you can just

take it to the store and become so much more healthy I'll put a link for you to click on, all

you need to do is just put in your e-mail address and first name

and I'll send you a grocery list of the best foods in the world that you can take to your natural grocery store and really really change your life

and my channe iss about quick nutritional videos to helpe remind you about health and energy

so make sure to subscribe to my Youtube channel so you get reminders on

what you can do to remain healthy and stay healthy as possible so thanks so much for your time and I wish you the best of health take care

bye bye

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