[AGAR RECIPE, backyard scan] It's January 2017... as I look out of our
backyard and get ready to feed the animals
before I complete the video on the
recipe to make agar in surgical
pathology for agar pre-embedding of
surgical path specimens.
[AGAR RECIPE, Dr. Ervin Shaw] I hope that youall enjoy your careers as much as I've
enjoyed mine... and... enjoy this series of
agar pre-embedding techniques.
[AGAR RECIPE, encounter with Dr. Dimenstein] First, I would like to thank doctor [M. D., Ph.D]
Izak B. Dimenstein of Michigan [USA] for
contacting me by email in 2016 and
ultimately stimulating me to the desire
to produce videos of how to use agar
pre-embedding in a surgical pathology
practice. Uh... A... series of techniques that we
have used since 1977 and have added to
accuracy and efficiency for the "slide
diagnosing" pathologist.
[AGAR RECIPE, www.palpath.com recipe page] As you can see here... on our website... we have the recipe
for... making agar.
And then... here, we have...on the website... information about the
history of our use [of it] in a practice that
probably was doing 5,000 specimens a
year in 1977 when we started...
and... currently is in the low 30 thousands
of specimens. And we are still
very certain that this is a highly effective...
(1) cost effective and (2) accuracy effective
technique in our lab... We hope to share it
with you! Thank you!
[AGAR RECIPE, measuring agar for a batch] Okay, Brenda Speer is measuring out
the [dry] agar... 42 grams... and this plastic
specimen container is going to be what
it ultimately gets poured into... and we'll let
it solidify. [background chatter]
{Brenda: Take distilled water to it} Okay... there's the 42 grams [of dry agar].
[AGAR RECIPE, measuring distilled water for a batch] Now we are getting the distilled water... 1400 cc's......
measure'd out... & we're gonna add it to the container
with the dry agar... [background chatter]
and we've come back into another little area [adjacent processor room]
of surgical pathology
to mix
[AGAR RECIPE, mix it and microwave heat the mix] We're stirring it up with the wooden stick...
{Brenda: it's actually a tongue depressor} ...tongue depressor...
[mumbling]
Is that cold water or warm water? {Brenda: cold...now we're gonna put it in the microwave} So it's [mixed] with
cold water and now into the
microwave {Brenda: for 15 minutes...
DO NOT let it boil!} So you will do it for 15
minutes and you have to have practiced to
see what setting you put it on so that
it will not boil.
[AGAR RECIPE, re-liquify aliquot of stored solid agar] Now here's an example of the old solid block where
Brenda's cut up a piece... {Brenda: there's one peice right there.} put it into
a little plastic container like that...
{Brenda: Then what we do is heat this up... and I use...} Put this into there.
{Brenda: According to how much you're gonna be using.... [then]
how much you're gonna put in here. But you
gotta let it melt down... get hot... but not
boil.} So we've got to get it hot
Don't let it boil... This is when you do
your daily filling of the ... ... of the
[test] tubes in the heat blocks. So the microwave
and the argar are simply kept in their
containers over in this corner, ... at room
temperature. And the [test] tubes for the heat
block are filled daily ... replenished daily...
and then a batch of new agar is made up
every Tuesday for our busy surgical path
lab. [mumble] Okay...
the melted agar is taken out and we're gonna add Thymol... to it
...and Brenda says that
the Thymol throws of a pretty intense
smell ... so, stay away from it. {Brenda:Yeah, don't breath it in. I kinda back away from it.}
Stir it in a little bit...
and then after you've got set in,
you let it solidify? {Brenda: yep ...I just put the lid on it & stick it back in the corner.}
Once Brenda put it in & started the
microwave oven,
she set her cellphone timer. And, in our
lab... with this particular oven... it takes
15 minutes to cook the fresh agar batch.
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