Waching daily Nov 21 2017

(music)

(chiming)

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- Gene editing is making precise

and permanent changes to living things

at the level of the DNA-

the A's, T's, C's, and G's that are

the source code and the building blocks of DNA.

There are several ways to do targeted gene editing,

but the one everybody is talking about

right now, is CRISPER-Cas9.

We've taken the natural bacterial immune system

and tweaked it to be a highly precise pair

of molecular scissors that can be directed

to any particular sequence of A's, T's, C's, and G's.

CRISPER-Cas9 has a few ingredients:

a guide RNA, the Cas9 enzyme,

the DNA that we want to change,

and a short DNA that has the edit we want.

We synthesize a guide RNA that contains

the sequence that we want to edit.

For example, the DNA sequence that causes

a disease like sickle cell anemia.

We have to have the DNA in the living thing

that we want to edit.

For example, a chromosome in a human sperm or egg.

We mix these things with Cas9,

an enzyme that's shaped like a hand.

I just happen to have Cas9 on me.

This is Cas9.

Cas9 grabs the guide RNA, and then starts searching

the chromosomal DNA for a match.

When the guide RNA matches the DNA sequence,

Cas9 finds it and cuts the chromosomal DNA.

So Cas9 is both the hand and the scissors,

and the guide RNA guides the hand

to the right place to cut.

If we want to knock out a gene-

make it not work anymore-

we can stop right there.

But if we want to edit the gene,

we add one more ingredient:

DNA that contains the sequence we want to add in.

Adding that template DNA to the mix tricks

the cell into repairing its DNA

with the new sequence, so in this case,

the sequence that is healthy instead of

the old sequence, which we said was sickle cell anemia.

Now the cell's chromosome has the edited DNA sequence,

and will pass it on to every other cell when it divides.

If you've edited a sperm or an egg,

then every cell in the embryo,

and eventually the person,

will carry the new DNA sequence.

So is this easy?

Not really, but it's a lot easier

than any other way we've had to edit DNA so far.

(bright music)

(metallic chiming)

(metallic whooshing)

For more infomation >> What Is Gene Editing? | Tech-x-planations | Singularity University - Duration: 2:30.

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Aston Martin Vulcan is a BEAST - Pcars2 [Mixed Reality / Oculus VR] - Duration: 10:09.

Kajot2111: It's getting brighter

really? ... Kajot2111: yes

Then I have to drive propperly now

Kajot2111: When you look after this corner it looks brighter than last lap

Kajot2111: Come here dude!

Oh yes

I change the camera quickly

For more infomation >> Aston Martin Vulcan is a BEAST - Pcars2 [Mixed Reality / Oculus VR] - Duration: 10:09.

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America IS the only country, right Roberta?! | Family Feud - Duration: 1:28.

HEY, MISS ROBERTA,

WE GOTTA SLOW IT DOWN NOW.

WILKINS FAMILY CAN STEAL.

WE GOT TWO STRIKES.

A HUNDRED WOMEN,

"TELL ME A COUNTRY

WHERE YOU THINK

THE MEN ARE SEXIER

THAN AMERICAN MEN."

ROBERTA: UNITED STATES

OF AMERICA.

[CHEERING AND APPLAUSE]

AMERICA!

WHAT?

[LAUGHTER]

DAVE: WHAT WAS THAT QUESTION?

GREG: YEAH, REPEAT THE QUESTION,

STEVE.

ROBERTA: EDIT THAT PART OUT.

STEVE: WE ASKED 100 WOMEN,

"TELL ME A COUNTRY

WHERE YOU THINK

THE MEN ARE SEXIER

THAN AMERICAN MEN."

[LAUGHTER]

UNITED STATES OF AMERICA.

[LAUGHTER]

OH, YEAH.

I SEE EXACTLY WHERE THAT ANSWER

CAME FROM. WELL, GOOD.

[LAUGHTER]

STEVE: YOU CAN'T CHANGE IT,

BUT YOU WANT TO GO WITH

THAT ONE?

ROBERTA: BOY, THE LOOKS

I GOT WERE UNBELIEVABLE.

STEVE: YEAH.

YOU KNOW, WHEN YOUR SON

DOESN'T GO, "GOOD ANSWER, MOM--"

ROBERTA: YEAH, THAT'S BAD.

STEVE: YEAH, WELL--MOST SONS

WILL SUPPORT THEIR MOTHERS

NO MATTER WHAT.

ROBERTA: NOT THEM.

STEVE: YOU SAID THAT,

AND YOUR BOY WENT,

"DAMN, MAMA."

[LAUGHTER]

UNITED STATES OF AMERICA!

For more infomation >> America IS the only country, right Roberta?! | Family Feud - Duration: 1:28.

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[ENG SUB] Because This Is My First Life EP. 15 Preview | This Life is Our First | 이번 생은 처음이라 - Duration: 2:08.

For more infomation >> [ENG SUB] Because This Is My First Life EP. 15 Preview | This Life is Our First | 이번 생은 처음이라 - Duration: 2:08.

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(November 21, 2017) A woman says her mother's cruelty is taking a toll on their relationship - Duration: 42:56.

For more infomation >> (November 21, 2017) A woman says her mother's cruelty is taking a toll on their relationship - Duration: 42:56.

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This Is How a Victoria's Secret Fashion Show 2017 Costume Gets Made - Duration: 2:45.

My name is Barak Stribling

and we are in my shop

In Jersey City, New Jersey

This is our 8th Victoria Secret Fashion Show

We typically spend a lot of time working on this show

there are several fittings that happen

throughout the summer

and then once we hit the 2 weeks prior to the filming of the show

they have major fittings for all the girls

all the girls that have been cast

and so we will have to do alterations

change things to fit specific girls

because not everything is built for a specific girl

The process usually goes something like this

they will ask us over —

early in the year

typically

April, even as early as March to show us sketches

and let us know what they want us to built

then we will have out first fitting in April

until it gets to showtime

and through that time somethings will change

somethings, maybe they might add something

but every month we have a fitting

There's two moments that are especially gratifying

when working the show

When you see the garment that you've made

on the girl that it was meant to be on

there have been times when 8 girls have tried on the same garment

and it was like

she's not the one

that's not it

and then the 9th one will try it on

and you'll go

that's what it's suppose to look like!

and so that's a real thrill

and then the other thrill

is actually watching them

work the runway in the garment

that's one thing that I love about the Victoria Secret Fashion Show

You get to see the girl

It's not just about the product

they don't walk with this face

you know

down the runway

so serious

they have personalities

and strengths

and power

and you get to see every bit of it

when they walk the runway

so we're just frosting really

but you know

I mean, it's fun frosting

because we get to make beautiful garments

for beautiful women

what's to hate

it's great